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Representative single confocal images (A, C & E) or serial Z-stacks (B, D & F) of MCF-12A cells embedded in 50% (A & B), 75% (C & D) or 100% (E & F) Matrigel and immunolabeled with an E-cadherin antibody (green). Nuclei are stained with 4′,6-diamidino-2-phenylindole (blue). Scalebars: 100 μm.
