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. 2018 Jul 2;4(7):FSO317. doi: 10.4155/fsoa-2018-0022

Figure 1. . RACK1 turnover is influenced by ubiquitin–proteasome system.

Figure 1. 

Endogenous RACK1 expression in HEK 293 cells was evaluated by western blot following a time course of (A) 50 μg/ml cycloheximide and (B) 20 μM MG132. Bar charts in lower panels represent the mean of three independent experiments. (C) RACK1 was immunoprecipitated from control HEK 293 cell lysate or lysate that had been extracted from cells pretreated with MG132. Immunoprecipitates were blotted for either RACK1 (upper panel) or ubiquitin (lower panel). (D) HEK 293 cells were transfected with HA-tagged ubiquitin and Myc-tagged RACK1 and treated with MG132. Myc-RACK1 was immunoprecipitated from cell lysates and blotted for HA-ubiquitin (upper panel). Cell lysates were blotted for HA (lower panel).

HA: Human influenza hemagglutinin.