Table 1. . Virulence genes and primers used for quantitative RT-PCR detection.
| Gene | Function | Sequence of primer† | Size of product (bp) |
|---|---|---|---|
| hla | α-hemolysin | F: 5′-AATGAATCCTGTCGCTAATGCCGC-3′ | 269 |
| R: 5′-CTGAAGGCCAGGCTAAACCACTTT-3′ | |||
| hld (RNA III) | δ-hemolysin/gene expression regulator RNA III | F: 5′-TAATTAAGGAAGGAGTGATTTCAATG-3′ | 100 |
| R: 5′-TTTTTAGTGAATTTGTTCACTGTGTC-3′ | |||
| agrA | Regulator of gene expression | F: 5′-TGATAATCCTTATGAGGTGCTT-3′ | 164 |
| R: 5′-CACTGTGACTCGTAACGAAAA-3′ | |||
| arlS | Regulator of gene expression | F: 5′-TGGAATACCAATTCCATGATCT-3′ | 103 |
| R: 5′-TGCAATCAAATATGATGTGAAGAA-3′ | |||
| srrA | Regulator of gene expression | F: 5′-AGCATGTGTGGGAGGTATGA-3′ | 118 |
| R: 5′-CCTCTTGGCCATTACTTGCTT-3′ | |||
| saeS | Phosphorylated response regulator | F: 5′-ATCCGAACAACAAGAAAAAACAG-3′ | 110 |
| 5′-TGATTATACCATCACGTAGTCCTTCA-3′ | |||
| yycG | Regulator of gene expression | F: 5′-TACAATCCCTTCATACTAAACTTGTAATTG-3′ | 198 |
| R: 5′-GTGCATTTACGGAGCCCTTTTCGTCATATAC-3′ | |||
| sasG | Surface protein | F: 5′-GGTTTTCAGGTCCTTTTGGAT-3′ | 192 |
| R: 5′-CTGGTGAAGAGCGAGTGAAA-3′ | |||
| spa | Surface protein for bacterial aggregation | F: 5′-GCGCAACACGATGAAGCTCAACAA -3′ | 125 |
| R: 5′-ACGTTAGCACTTTGGCTTGGATCA-3′ | |||
| clfA | Surface protein | F: 5′-CGGTTTTGGACTACTCAGCA-3′ | 151 |
| R: 5′- GCTACTGCCGATAAACTA-3′ | |||
| fnbA | Surface protein | F: 5′-ACTTGATTTTGTGTAGCCTTTTT-3′ | 185 |
| R: 5′-GAAGAAGCACCAAAAGCAGTA-3′ | |||
| fnbB | Surface protein | F: 5′-CGTTATTTGTAGTTGTTTGTGTT-3′ | 118 |
| R: 5′- TGGAATGGGACAAGAAAAAGAA-3′ | |||
| icaA | PIA or PNAG production | F: 5′-AACAGAGGTAAAGCCAACGCACTC-3′ | 85 |
| R: 5′-CGATAGTATCTGCATCCAAGCAC-3′ | |||
| sigB | Regulator of gene expression | F: 5′-TCAGCGGTTAGTTCATCGCTCACT-3′ | 156 |
| R: 5′-GTCCTTTGAACGGAAGTTTGAAGCC-3′ | |||
| codY | Regulator of gene expression | F: 5′-AAAGAAGCGCGCGATAAAGCTG-3′ | 120 |
| R: 5′-TGCGATTAATAGGCCTTCCGTACC-3′ | |||
| sarA | Regulator of gene expression | F: 5′-CCTCGCAACTGATAATCCTTATG-3′ | 173 |
| R: 5′-ACGAATTTCACTGCCTAATTTGA-3′ | |||
| spoVG | Modulator of gene expression | F: 5′-TGTTCGTTGCAATGCCAAGT-3′ | 51 |
| R: 5′-TGTCGCGGAATTCACCATC-3′ | |||
| rot | Transcriptional regulator | F: 5′-AAGAGCGTCCTGTTGACGAT-3′ | 126 |
| R: 5′-TTTGCATTGCTGTTGCTCTA-3′ | |||
| gyrA | DNA gyrase A subunit/internal standard gene | F: 5′-CATTGCCAGATGTTCGTGAC-3′ | 117 |
| R: 5′-CACCAACGATACGTGCTGAT-3′ | |||
†The sequence of primers was designed based on the genome sequence of Staphylococcus aureus strain MW2 (Accession: BA000033.2).
bp: Base pair; PIA: Polysaccharide intercellular adhesion; PNAG: Polymeric N-acetylglucosamine.