Table 1. . Primary antibodies used in the evaluation of medulloepithelioma.

Antibody	Expression profile	Supplier	Type	Dilution
CD56	NCAM neural stem cell, natural killer cells	Cell Marque Corp, CA, USA	RM	1:2500
CD99 (12E7)	Ewing sarcoma/PNET marker, T-cell adhesion	Dako North America, CA, USA	MM	1:100
c-kit	Cell-surface receptor for the cytokine KIT/stem cell factor. Plays role in hematopoiesis, stem cell maintenance, gametogenesis, mast cell development and melanogenesis	Dako North America	RP	1:250
Glypican-3	Heparin sulfate glycoprotein that regulates events in embryogenesis, cancer	BioMosaics, VT, USA	MM	1:20
LIN28A (A177)	RNA binding protein suppressing let-7	Cell Signaling, Inc., MA, USA	RP	1:100
Nestin	Intermediate filament in neural stem cells	EMD Millipore, MA, USA	RP	1:200
OCT4	Marker of pluripotency, expressed in some germ cell tumors	Cell Marque Corp	MM	1:100
PAX8	Homeobox transcription factor important in eye/retinal development	Proteintech Group, IL, USA	RP	1:100
Pancytokeratin	Cytoskeletal proteins found in most epithelial cells	Ventana Medical Systems, AZ, USA	MM	1:100
PGP9.5	Marker of neuroblasts/neuroblastic differentiation, oocytes	Vector Laboratories, CA, USA	MM	1:25
SALL4	Transcription factor of SALL family; regulator of OCT-4 expression; expressed in germ cell tumors and yolk sac tumors	Biocare Medical, CA, USA	MM	1:10
SOX2	Maintenance of pluripotency; expressed in germ cell tumors, some lung carcinomas, glioblastoma	Cell Signaling Technology	MM	1:15
Synaptophysin	Neurosecretory vesicles; expressed in synapses, neuroendocrine tumors	Ventana Medical Systems	RM	Predilute
S100	Calcium binding protein; expressed in variety of tissues including neural, histiocytes	Vector Laboratories	RP	1:1000
Vimentin	Class III intermediate filament expressed in cells of mesenchymal origin	Ventana Medical Systems	MM	Predilute

MM: Mouse monoclonal; PNET: Primitive neuroectodermal tumor; RM: Rabbit monoclonal; RP: Rabbit polyclonal.