Fig. 2. (a) Sequence of the Pf 26mer Box C/D RNA. (b and c) Expansions of the ribose C1′–H1′ and base C6/8–H6/8 regions of 13C–1H CP-HSQC spectra recorded on a 1 GHz spectrometer and 111 kHz MAS. In (b) two resonances in the dotted box correspond to C4′–H4′ correlations, and the horizontal dashed line at approx. 90 ppm 13C separates C1′–H1′ resonances of nucleotides located in helical and non-helical regions. The signals are labeled according to the 1H assignment determined in this work and the previously reported sequence specific 13C,15N assignment.2 The asterisk indicates resonances of non-assigned nucleotides.
