Reagent/Resource	Reference or Source	Identifier or Catalog Number
Experimental Models
MCF10A pBabePuro (Homo sapiens)	Zev Gartner Lab	n/a
MCF10CA1d.cl1 (H. sapiens)	Barbara Ann Karmanos Cancer Institute	n/a
Antibodies
CENP‐E (1:400, mouse monoclonal, clone 1H12)	Abnova	MAB1924
BubR1 (1:600, mouse monoclonal, clone 8G1)	Thermo Fisher	MA5‐16036
beta‐Tubulin (1:5,000, mouse monoclonal, clone TUB 2.1)	Abcam	ab11309
CDC20 (1:400, rabbit polyclonal)	Bethyl	A301‐179A
gamma‐Tubulin (1:12,000, rabbit polyclonal)	Abcam	ab176404
LC3A (1:400, rabbit polyclonal)	Novus	NB100‐2331
Survivin (1:1,000, rabbit monoclonal, clone EP2880Y)	Abcam	ab176402
INCENP (1:1,000, mouse monoclonal, clone 3D2)	Thermo Fisher	MA5‐17100
Aurora A (1:6,000, rabbit monoclonal, clone EPR5026)	Abcam	ab176375
CENP‐A (1:500, rabbit polyclonal)	Abnova	PAB18324
HMGB1 (1:3,000, rabbit monoclonal, clone EPR3507)	Abcam	ab176398
γ‐H2AX (1:2,500, rabbit monoclonal, clone 20E3)	Cell Signaling	9718BF
Chemicals, enzymes and other reagents
Matrigel
Matrigel, growth factor reduced, phenol red‐free	Corning	356231
Antibody conjugation
DyLight 550 Microscale labeling kit	Thermo Fisher	84531
DyLight 650 Microscale labeling kit	Thermo Fisher	84536
Medium
DMEM/F12, no phenol red	Gibco	21041‐33
Horse Serum	Gibco	16050‐122
EGF	Sigma	E9644‐.2MG
Hydrocortisone	Sigma	H0888‐1g
Cholera Toxin	Sigma	C8052‐1MG
Insulin	Life Technologies	12585014
Drugs
Barasertib (1.11 nM)	SelleckChem	S1147
CHR‐6494 (500 nM)	MedChem Express	HY‐15217
CW069 (25.0 μM)	SelleckChem	S7336
Etoposide (333 nM)	SelleckChem	S1225
GSK461364 (2.20 nM)	SelleckChem	S2193
GSK923295 (3.20 nM)	SelleckChem	S7090
Ispinesib (1.70 nM)	SelleckChem	S1452
MK‐5108 (0.576 nM)	SelleckChem	S2770
MK‐8776 (9.00 nM)	SelleckChem	S2735
Paclitaxel (2.67 nM)	SelleckChem	S1150
Vinblastine (2.40 nM)	Sigma	V1377
YM155 (0.540 nM)	SelleckChem	S1130
Software
R	https://r-project.org	3.3.3
Knime	https://www.knime.com	3.2.1
Python	https://python.org	3.4
Zeiss Zen blue	http://zeiss.com	2012
Zeiss Zen black	http://zeiss.com	2012
LabView	http://ni.com	2013 SP1
Fiji/ImageJ	https://fiji.sc	fiji‐latest, timestamp 20160902160412
MATLAB	https://www.mathworks.com/	2015a
IPA	https://www.qiagenbioinformatics.com/products/ingenuity-pathway-analysis/
Other
Axio Observer.Z1	Carl Zeiss Microscopy	431007 9901 000
Scan Module LSM 710 Base Unit	Carl Zeiss Microscopy	000000 1410 052
Spectral Detection Unit LSM 780 + 2 PMT	Carl Zeiss Microscopy	000000 1670 619
Objective Plan‐Apochromat 20×/0.8 M27	Carl Zeiss Microscopy	420650 9901 000
Laser diode 405 nm CW 30 mW	Carl Zeiss Microscopy	000000 1410 119
Laser 561 nm	Carl Zeiss Microscopy	000000 1410 117
Master Beam Splitter Wheel for Laser 633, 561, 514, 488, 458 nm	Carl Zeiss Microscopy	000000 1583 982
Beam path NLO+405/445 Axio Observer/Axio Imager	Carl Zeiss Microscopy	000000 1741 731
Spinning Disc Unit CSU‐X1A 5000	Carl Zeiss Microscopy	423638 9090 000
Spinning Disc Laser Unit 405 nm	Carl Zeiss Microscopy	000000 1514 464
Rolera EM‐C2 Bio‐Imaging Microscopy Camera, Mono, 14‐bit	Carl Zeiss Microscopy	000000 1915 668
PROcellcare 5030 + Peristaltic Pump Extension Unit	PROdesign	n/a
Energenie EG‐PM2	Energenie	EG‐PM2
4× LabTek holder	EMBLEM	LTT‐01 and LTT‐02