Table 2.
Details of the primers used for quantitative real time PCR analysis of chicken mRNAs
| Target | Target Primers | PCR product (bp) | Accession No. |
|---|---|---|---|
| β-Actin | F:5’-AGCGAACGCCCCCAAAGTTCT-3’ | 139 | NM_205518.1 |
| R:5’-AGCTGGGCTGTTGCCTTCACA-3’ | |||
| FAS | F:5’-GCTGGCTACAGTGGTGGACT-3’ | 129 | NM_205155.2 |
| R:5’-CCACCTCGAACCACCAAAGC-3’ | |||
| LPL | F:5’-CTCCGATCCCGAAGCTGAGATG-3’ | 106 | NM_205282.1 |
| R:5’-CTGTCCAGGAACCAGGTAGC-3’ | |||
| ACC | F:5’-CAACGAGTCGGGCTACTACC-3’ | 146 | J03541.1 |
| R:5’-GGTCCTTGGTCACGTATGGG-3’ | |||
| MAE | F:5’-GGACCTCGAAGCCTTCATCC-3’ | 77 | AF408407.1 |
| R:5’-GCCAGGTGTATGAGTCAGCC-3’ | |||
| CAT | F:5′-TGGCGGTAGGAGTCTGGTCT-3′ | 112 | NM_001031215.1 |
| R:5′-GTCCCGTCCGTCAGCCATTT-3′ | |||
| SOD1 | F:5-CACTGCATCATTGGCCGTACCA-3′ | 223 | NM_205064.1 |
| R:5-GCTTGCACACGGAAGAGCAAGT-3′ |
Abbreviations: FAS fatty acid synthase, LPL lipoprotein lipase, ACC acetyl-coA carboxylase, MAE malic enzyme, CAT catalase, SOD1 superoxide dismutase 1