Fig. 5. oLOX can be used to image LOXF activity in a fibrotic ventilating ex vivo asinine lung model. Catheter installation of 200 μM oLOX instilled in a total volume of 1 mL in PBS into a whole fibrotic ventilating ex vivo asinine lung results in a significant increase in fluorescent signal (a and b, *p = 0.0313, Wilcoxon signed rank test, n = 3); (c) tissue shown in (a) was excised and evaluated for LOX expression using immunohistochemistry (20× lens). (d) Western blot confirmed expression of LOX in both fibrotic and grossly unaffected (control) areas of ex vivo asinine lung tissue.
