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. Author manuscript; available in PMC: 2019 Jun 15.
Published in final edited form as: ACS Chem Biol. 2018 May 17;13(6):1610–1620. doi: 10.1021/acschembio.8b00230

Figure 5.

Figure 5

Complementation of sulfide-induced growth phenotype and increased CoASSH levels in a ΔcstB S. aureus Newman strain by E. faecalis CoAPR. Representative growth curves for wild-type (WT), ΔcstB, and plasmid-complemented ΔcstB allelic S. aureus Newman strains on HHWm media in the presence of 0 mM and 0.2 mM NaHS added at t = 0 h. (a) WT (black squares), ΔcstB (blue squares), and ΔcstB:pCstB (red squares) strains in the presence of 0 mM NaHS; WT (black circles), ΔcstB (blue circles), and ΔcstB:pCstB (red circles) strains in the presence of 0.2 mM NaHS. (b) ΔcstB:pCoAPR (violet squares), ΔcstB:pCoAPRC42S (green squares), and ΔcstB:pCoAPRC508S (pink squares) strains in the presence of 0 mM NaHS; ΔcstB:pCoAPR (violet circles), ΔcstB:pCoAPRC42S (green circles), and ΔcstB:pCoAPRC508S (pink circles) strains in the presence of 0.2 mM NaHS. (c) Ratio of CoASSH to total CoA thiol for WT (black), ΔcstB (gray), ΔcstB:pCstB (red), and ΔcstB:pCoAPR (blue). Values represent mean ± SD of the mean derived from replicate experiments with statistical significance established using a paired t-test relative to wild-type strain under the same conditions (**p ≤ 0.01, *p ≤ 0.05).