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. Author manuscript; available in PMC: 2018 Oct 1.
Published in final edited form as: Biol Blood Marrow Transplant. 2017 Jul 8;23(10):1622–1630. doi: 10.1016/j.bbmt.2017.06.018

Figure 2. Reduced STAT3 expression in host myeloid APCs amplifies the severity of acute GVHD.

Figure 2

Host B6-WT and B6-LysM/Cre STAT3fl/− animals were lethally irradiated (11 Gy) on day −1 and infused with 2 × 106 CD90.2+ T cells along with 5 × 106 bone marrow (BM) cells from either syngeneic B6 or allogeneic BALB/c animals on day 0. (A) Survival (n=12–16 per group). Data are combined from three experiments with similar results. (B) GVHD clinical score. (C) Representative hematoxylin and eosin (H&E) stained images of liver on day 14 after allo-BMT are shown. (D and E) Histopathological GVHD scores from liver (D) and gastrointestinal (GI) tract (E) on day 14 after allo-BMT (n=12 per group, pooled from two experiments). Balb/c-WT mice were irradiated (8 Gy) and then immediately i.p. injected with 20 mg/Kg WP1066 STAT3 inhibitor or diluent. At 24 and 48 hours after injections mice were euthanized and whole spleens (F) and peritoneal (G) macrophages and were harvested (n=3 per group). Cells were analyzed for CD11b+ cells within the myeloid gate. All error bars show the mean ± SEM. **p<0.01, *p<0.05.