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. Author manuscript; available in PMC: 2018 Aug 13.
Published in final edited form as: Expert Rev Mol Diagn. 2018 Jan 16;18(2):155–163. doi: 10.1080/14737159.2018.1427068

Table 4.

Currently used methods to detect AR-V7 in tumor and liquid biopsies

Tissue-based AR-V7 detection methods
- Western blot for protein detection53
- IHC for protein detection54
- RNA in situ hybridization (RISH) for mRNA5557
- RT PCR for mRNA detection58
- RNA sequencing for mRNA detection59
Blood-based AR-V7 detection methods
- CTC based RT PCR for mRNA detection31
- CTC based ddPCR for mRNA detection60
- CTC based RNA-seq61
- CTC based protein (immunofluorescence] detection34
- Whole blood analog RT PCR for mRNA detection38
- Whole blood ddPCR for mRNA detection39
- Cell free RNA detection by RT-PCR62
- Exosome derived PCR detection of mRNA63

Abbreviations: AR-V7: androgen receptor splice variant 7; IHC: immunohistochemistry; RT PCR: reverse transcription polymerase chain reaction; mRNA: messenger ribonucleic acid.