Table 4.
Tissue-based AR-V7 detection methods |
- Western blot for protein detection53 - IHC for protein detection54 - RNA in situ hybridization (RISH) for mRNA55–57 - RT PCR for mRNA detection58 - RNA sequencing for mRNA detection59 |
Blood-based AR-V7 detection methods |
- CTC based RT PCR for mRNA detection31 - CTC based ddPCR for mRNA detection60 - CTC based RNA-seq61 - CTC based protein (immunofluorescence] detection34 - Whole blood analog RT PCR for mRNA detection38 - Whole blood ddPCR for mRNA detection39 - Cell free RNA detection by RT-PCR62 - Exosome derived PCR detection of mRNA63 |
Abbreviations: AR-V7: androgen receptor splice variant 7; IHC: immunohistochemistry; RT PCR: reverse transcription polymerase chain reaction; mRNA: messenger ribonucleic acid.