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. 2001 Oct 23;98(23):13431–13436. doi: 10.1073/pnas.231324698

Figure 4.

Figure 4

Northern blot analysis of RNA from roots of different cereals. Probes were AsCS1 (CS) and AsbAS1 (βAS). Ten micrograms of total RNA was loaded per lane. RNA levels were monitored by using methylene blue (MB) dye. Hybridization was performed in Church buffer (500 mM phosphate buffer/7% SDS/1 mM EDTA/1% BSA) for 16 h at 65°C. The filters were washed with 40 mM phosphate buffer, 5% SDS, and 1 mM EDTA (3 × 15 min), followed by 40 mM phosphate buffer, 2% SDS, and 1 mM EDTA (3 × 15 min), and then 40 mM phosphate buffer, 1% SDS, and 1 mM EDTA (2 × 15 min). All washes were carried out at 65°C. Lane 1, A. strigosa S75 (21); lane 2, A. longiglumis (33); lanes 3 and 4, A. strigosa accession nos. CI1994 and CI13815 (40); lane 5, Triticum aestivum (cultivar Riband); lane 6, Hordeum vulgare (cultivar Golden Promise); lane 7, O. sativa (accession M12); and lane 8, Zea mays (accession P10).