Table 1.
Comparison of MF methods used to detect C. perfringens spores in water
| Comparison | Accuracy | Specificity | Disadvantages | Heat treatment | Reference |
|---|---|---|---|---|---|
| Egg yolk-free TSC vs mCP | TSC >mCP | mCP >TSC; both > 90% | Overlaying the MFs with TSC did not improve recovery and sometimes caused blackening in the overlays | 75 °C, 20 min | Sartory (1986) |
| mCP vs MmCPa | mCP: 95% ± 4 MmCP: 94% ± 4 | No difference | None reported | Not done | Armon & Payment (1988) |
| TSC vs mCP | TSC: 82.5%, mCP: 60% | mCP more selective than TSC | Poor color differentiation on mCP following exposure to ammonia fumes | 75 °C, 20 min | Sartory et al. (1998) |
| TSCFb vs mCP | TSCF: 80.8%, mCP: 53.5% | TSC:14.8% false +, 17.2% false −; mCP: 41.6% false +, 1.9% false− | mCP preparation was complex and mCP was cumbersome to use | Not done | Araujo et al. (2001) |
| TSC vs TSCF vs mCP | TSC: 58%; TSCF: 88.5%; mCP: 58.2% | TSCF is more specific for spore recovery | mCP performed poorly if water samples contained predominantly spores | 80 °C, 5 min | Araujo et al. (2004) |
| TSC vs TSC-EYc vs mCP | TSC >TSC-EY >mCP | false +: mCP 6%; TSC: 74% TSC-YE: 76% | mCP was labor intensive and time consuming | Not done | Burger et al. (1984) |
| FDTd vs CP AnaSelecte vs mCP vs SFP | All were equivalent | CP AnaSelect more selective than mCP | CP AnaSelect slower (12–15 hr) than FDT (5–6 hr) | 70 °C, 2.5 min | Vijayavel et al. (2009) |
| CCPf vs mCP | CCP >mCP | CCP more specific than mCP | Poor color differentiation on mCP following exposure to ammonia fumes | Not done | Manafi et al. (2013) |
a
Modified mCP (MmCP) with indoxyl-β-D-glucoside reduced from 600 to 60 mg/L.
b
Fluorogenic TSC supplemented with 4-methylumbelliferylphosphate (MUP) disodium salt.
c
TSC agar without egg yolk.
d
Fung double tube method.
e
CP AnaSelect Oxyplate (Oxyrase Inc., Mansfield, OH).
f
CP Chromo Select Agar (CCP Sigma-Aldrich).