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Loss of intraocular NPC over 6 weeks at varied zeta potential. Decay of intraocular fluorescence at 3 and 6 weeks, at and beyond postinjection day 10 (“day 0”) is seen in individual eyes injected with Cy7-tagged 3R-NPC (top row), 2R-NPC (middle row), and unconjugated NP (bottom row). Spreading and loss of fluorescence is slowed in more highly charged NPC, with correspondingly greater zeta potential.
