Figure 7.

Early clearance of IVT injected Cy7-CDEX-2R, at elevated total dose. (A) In vivo loss of Cy7-CDEX-2R from rabbit vitreous is shown as photons/s (normalized to cm² in 3.5 cm² circles) for 3 distinct doses, one being the dose (30 μL at 3 mg/mL, 90 μg total) described in Figure 6C. IVIS scans were taken at day 10, 24, 31, and 38. Cy7-CDEX-2R (30 μL) was from stocks at 4 different concentrations: control, 0 mg/mL (n = 3); 3 mg/mL (n = 3); 6 mg/mL (n = 2); and 12 mg/mL (n = 2). Samples at 3 mg/mL and 6 mg/mL (180 μg total) gave proportional day 10 photons/second emission. NPC at 12 mg/mL (360 μg total dose) was much lower at the earliest scan, even below the 4X lower dose. (B) Later time points are shown as percent emission remaining compared to day 10 (taken as “day 0”, 100%). Half-life of residual NPC loss, after day 10, at the two lower doses, is similar to that observed previously. The residual loss, at the highest dose is slower.