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. 2018 Jun 4;7(8):3548–3560. doi: 10.1002/cam4.1589

Figure 4.

Figure 4

Knockdown of FLI‐1 inhibits clonogenicity and cancer stem cell properties in vitro and suppresses tumorigenesis in vivo. A, The colony‐forming assay demonstrated that the knockdown of FLI‐1 reduced the cell colony‐forming number. B, The knockdown of FLI‐1 decreased the expression of mammary stem cell markers (ALDH1 and CD133) compared with those of the control group. The overexpression of FLI‐1 in the MCF‐7 cells upregulated the expression of stem cell markers. C, In the shFLI‐1 group of the MDA‐MB231 cells, the sphere‐forming ability was attenuated. The overexpression of FLI‐1 in the MCF‐7 cells upregulated the sphere‐forming capacity. Each bar represents mean ± standard deviation (SD). *P < .05. D, The images of excised tumors 30 days after the orthotopic injection of tumor cells into the mammary gland fat pad. The statistical analyses of tumor weight and tumor diameter between the two groups. Each bar represents mean ± SD. *P < .05. E, The representative images of tumor sections stained with hematoxylin and eosin, anti‐FLI‐1, and anti‐ALDH1. The statistical analysis of the expression of ALDH1 between the two groups. Each bar represents mean ± SD. *P < .05