Figure 2.

Verification that OCT4 positively regulated LEF1 expression in Eca109 cells. A, The transfection efficiency of the lentiviral system in Eca109 cells, evaluated by fluorescence microscopy, reached ≥90% (scale bar = 50 μm). B, Relative mRNA expression level was significantly decreased in the LV‐shOCT4 group by two‐tailed t test (n = 3 for each cell group). C, Protein levels of OCT4 and LEF1 in the LV‐shOCT4 group were significantly downregulated by two‐tailed t test. D, Analysis of gray level between each group was applied by ImageJ software. All experiments were performed in triplicate