Figure 3.

LEF1 was a facilitator of OCT4 in regulating the EMT, invasion, and migration of Eca109 cells. A, Relative mRNA expression level: successful overexpression of LEF1 in the LV‐shOCT4 group; overexpression of N‐cadherin and low expression of E‐cadherin in the p‐LEF1 group, two‐tailed ANOVA was used and LSD test was significant between shOCT4 and p‐LEF1 groups. B and C, The protein level in the p‐LEF1 group enhanced expression of N‐cadherin and decreased expression of E‐cadherin. Analysis of gray level was performed by ImageJ software, two‐tailed ANOVA was used, LSD test was significant between shOCT4 and p‐LEF1 groups, and LSD test was significant between shOCT4 and p‐LEF1 groups