Figure 6.

Mutation frequency validation and redefinition of true MSI target genes. (A) Mutation frequency of outlier mutations observed in the present exome cohort and in the TCGA cohort. (B) Mutation frequency for the 9 outlier mutations coding microsatellites contained in the putative target genes for MSI in a large independent cohort of colon tumors (n = 180) and the TCGA cohort (n = 53). (C) Distribution of mutation frequencies in coding microsatellites according to repeat length (x-axis). Genes with coding exonic outlier mutations identified in this study are indicated in green (positively selected) and blue (negatively selected). Genes with background mutation rates (unselected according to the model) are indicated in black. Examples of published targets for MSI are shown within rectangles, and those not subject to selection are indicated in black text.