Table 2. Technical and biological factors associated with the microbial structure of shrimp microbiota.

The impact was measured using Anosim (R value) and PERMANOVA with the adonis function (F and R₂ values) of Unweighted UniFrac distances. For each analysis we performed 1,000 permutations to obtain the p value.

Unweighted UniFrac
	Parameter	ANOSIM		PERMANOVA
		R	P value	pseudo-F	P value	R2	P value
Technical factors	Paper	0.970	0.001	19.866	0.001	0.667	0.001
	Primer	0.663	0.001	12.346	0.001	0.459	0.001
	Hypervariable region	0.659	0.001	14.482	0.001	0.410	0.001
	Country	0.522	0.001	10.753	0.001	0.341	0.001
	Sequencer	0.493	0.001	16.097	0.001	0.231	0.001
Biological factors	Lifestyle	0.333	0.001	8.630	0.001	0.139	0.001
	Organ	0.261	0.001	6.998	0.001	0.252	0.001
	Developmental stage	0.215	0.001	5.075	0.001	0.126	0.001