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. Author manuscript; available in PMC: 2018 Aug 13.
Published in final edited form as: Methods Mol Biol. 2018;1665:317–340. doi: 10.1007/978-1-4939-7271-5_17

Figure 2.

Figure 2

A screen shot from the microscope control program with regions of interest (boxes) superimposed on a dark field image of 320 nm, tethered particles. The cursors update continuously within the stationary boxes in the live image.