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. Author manuscript; available in PMC: 2019 Aug 15.
Published in final edited form as: Dev Biol. 2018 Apr 19;440(2):64–73. doi: 10.1016/j.ydbio.2018.04.010

Figure 5.

Figure 5

At E12.0 muscle, nerve, vasculature, and PPF-derived connective tissue and tendon progenitors are present in developing diaphragm region. (A–C, E, G–R) Into PPFs (as shown by GFP+ Prx1-derivatives in Prx1CreTg/+;Rosa26mTmG/+), muscle progenitors (Pax7+MyoD+ via immunofluorescence) migrate from the somites and phrenic nerve axons (Neurofilament+ via immunofluorescence) extend from the neural tube. Muscle progenitors and phrenic axon tip completely surrounded by PPFs. (D, F) Also at E12.0 vasculature (CD31+ via immunofluorescence) beginning to develop in PPF region in close association with muscle progenitors. cv, cardinal vein; vc, vena cava. Scale bar A-F = 200 um. Scale bar G-R = 200um. 3-dimensional movies in Supplemental Movies 8–12.