Figure 3. Iron mimics NGAL effects on chemokines expression in BHT101 cell lines.

Analysis of chemokines expression by qRT-PCR after treatment of NGAL-depleted BHT101 cell lines with FeCl₃ (A), and after the addition of the iron chelator DFO to the culture medium of parental and scrambled BHT101 cells (B). Data represent mean (± standard deviation) of three technical replicates within a single experiment. Each experiment has been repeated at least three times. Statistical analysis by unpaired Student’s t-test: ^*, p<0.0001; ^**, p<0.00001