Table 2. Trabecular bone phenotypes by microCT of the right distal femur of the mice*.
| Group, injected with | Sex | BMD (mg/cm3) | BV/TV (%) | Tb.Th (μm) | Tb.N (1/mm) | Tb.Sp (μm) | SMI | Conn.D (TV/mm3) |
|---|---|---|---|---|---|---|---|---|
| Prevention Study (Set 1): | ||||||||
| KO, saline | M | 988.1 ± 8.2 | 8.9 ± 1.1 | 33.6 ± 1.9 | 4.7 ± 0.1 | 207.5 ± 7.2 | 2.3 ± 0.2 | 222.6 ± 34.7 |
| KO, saline | F | 963.4 ± 5.2 | 5.2 ± 0.4 | 29.7 ± 0.2 | 4.0 ± 0.1 | 246.4 ± 3.0 | 2.7 ± 0.1 | 124.0 ± 30.4 |
| KO, 0.01× ETD | M | 1064.5 ± 30.9+ | 9.8 ± 1.3 | 34.1 ± 0.6 | 5.3 ± 0.1+ | 212.1 ± 18.0 | 2.1 ± 0.1 | 250.7 ± 46.5 |
| KO, 0.01× ETD | F | 999.5 ± 7.1+ | 10.2 ± 1.2+ | 33.3 ± 1.5 | 4.9 ± 0.3+ | 197.3 ± 12.3+ | 2.0 ± 0.2+ | 282.5 ± 37.9+ |
| KO, 0.12× ETD | M | 1023.4 ± 1.8+ | 18.0 ± 1.5++ | 36.5 ± 0.7 | 6.0 ± 0.2++ | 156.6 ± 6.9++ | 1.1 ± 0.2+ | 402.0 ± 28.0+ |
| KO, 0.12× ETD | F | 1146.6 ± 67.0+ | 15.1 ± 1.6++ | 34.4 ± 1.2+ | 5.5 ± 0.5+ | 177.5 ± 16.2+ | 1.4 ± 0.2++ | 400.6 ± 73.9+ |
| Reversal Study (Set 2): | ||||||||
| KO, saline | M | 993.1 ± 7.5 | 8.7 ± 1.2 | 35.2 ± 2.5 | 4.3 ± 0.1 | 224.9 ± 3.8 | 2.1 ± 0.2 | 169.5 ± 13.8 |
| KO, saline | F | 981.0 ± 2.6 | 3.4 ± 0.3 | 33.7 ± 1.1 | 2.9 ± 0.1 | 345.2 ± 10.0 | 2.9 ± 0.1 | 53.6 ± 8.5 |
| KO, NT | M | 994.4 ± 9.2 | 12.8 ± 3.4 | 41.6 ± 5.0 | 4.4 ± 0.2 | 219.0 ± 13.0 | 1.7 ± 0.3 | 166.1 ± 8.4 |
| KO, NT | F | 988.0 ± 11.2 | 4.2 ± 0.5 | 32.7 ± 1.2 | 3.2 ± 0.1 | 316.7 ± 12.5 | 2.5 ± 0.1 | 81.1 ± 8.4 |
| KO, 0.12× ETD | M | 1033.2 ± 10.5+ | 20.4 ± 2.7 | 44.6 ± 1.1 | 5.2 ± 0.3 | 184.1 ± 13.3 | 0.8 ± 0.2 | 220.4 ± 16.3+ |
| KO, 0.12× ETD | F | 982.1 ± 11.3 | 12.5 ± 1.2++ | 38.9 ± 1.4+ | 3.9 ± 0.1++ | 250.3 ± 6.1++ | 1.3 ± 0.1++ | 167.5 ± 11.5++ |
| WT, NT | M | 1003.8 ± 10.2 | 13.6 ± 0.1 | 48.2 ± 1.0 | 4.2 ± 0.2 | 226.2 ± 8.8 | 1.7 ± 0.2 | 140.2 ± 11.8 |
| WT, NT | F | 1003.3 ± 1.6 | 3.8 ± 0.8 | 34.8 ± 0.4 | 3.0 ± 0.2 | 335.0 ± 19.8 | 2.8 ± 0.1 | 59.5 ± 17.9 |
*For a description of different groups, see Table 1. Values are expressed as mean ± SE (three mice of each sex per group). Statistical significance in comparison to age-matched untreated KO mice of the same sex is indicated: +p < 0.05, ++p < 0.01. BMD, bone mineral density (mg/cm3); BV/TV, relative bone volume (%); Tb.Th, trabecular thickness (μm); Tb.N, trabecular number (1/mm); Tb.Sp, trabecular separation (marrow thickness, μm); SMI, structure model index; Conn.D, connectivity density (TV/mm3). ETD, etidronate; M, male; F, female; NT, not treated