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. Author manuscript; available in PMC: 2018 Aug 13.
Published in final edited form as: Comput Biol Chem. 2009 Dec 6;34(1):19–33. doi: 10.1016/j.compbiolchem.2009.11.002

Fig. 6.

Fig. 6

Estimated kinetic parameters Kb, Kt obtained by fitting MG model predictions to a family of stopped-flow data for range of Ca2+ concentrations by the DLS, quasi-Newton, and simulated annealing (SA) parameter estimation methods. Actin concentration is in excess of S1 ([A] = 2.5 μM, [S1] = 0.25 μM). In all simulations K1 and K2 are taken to be 0.25 μM−1 and 170, respectively. Backward rate constants are taken to be the same as denoted in Fig. 1. (A) Kb obtained by all three estimation methods displays sigmoidal dependence with Ca2+ concentration and sharply decreases for pCa<5.4 up to pCa = 6.6; (B) sigmoidal dependence Kt on pCa is less prominent having a scattered pattern for 5.4 > pCa>6.0 and differs little between the estimates obtained by different methods.