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. Author manuscript; available in PMC: 2018 Aug 13.
Published in final edited form as: Bio Protoc. 2016 May 20;6(10):e1819. doi: 10.21769/BioProtoc.1819

Table 1.

DI-PCR program

Program Temperature (°C) Time
50 2 min Hold
Denaturation 95 10 min Hold
Denaturation 95 5 sec 40 cycles
Annealing 55 25 sec
Extension 60 35 sec
Denaturation 95 15 sec Melting curve
Annealing 65 1 min
Denaturation 95 15 sec