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. 2018 Aug 1;7:e35898. doi: 10.7554/eLife.35898

Figure 1. COMMD proteins dimerize through the C-terminal COMM domain.

(A) Cartoon representation of COMMD proteins, and the MALLS profile of Commd1 (black), Commd7 (red) and Commd9 (blue) showing Commd proteins are dimers in solution (B) Comparison of the theoretical molecular weight of the COMMD proteins and the experimentally measured molecular weight. (C) MALLS analyses of the COMM and HN domain shows that the protein dimerisation occurs through the C-terminal COMM domain of the COMMD proteins as represented schematically. (D) Ribbon representation of the dimeric Commd9 COMM domain (residues 115–198) chain A (yellow) and chain B (blue). (E) The structure of the COMM domain dimer is analogous in orientation to a left-handed handshake.

Figure 1.

Figure 1—figure supplement 1. Schematic representations of bacterial expression constructs used in this study.

Figure 1—figure supplement 1.