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. Author manuscript; available in PMC: 2018 Aug 13.
Published in final edited form as: Methods Cell Biol. 2015 Apr 8;128:165–200. doi: 10.1016/bs.mcb.2015.02.007

Figure 5: Membrane-remodeling complexes visualized with negative stain versus cryo-electron microscopy.

Figure 5:

EndophilinA1 (A and D), Dynamin1 (B and E), or the hetero-complex formed by both proteins (C and F). In all cases, the purified proteins remodel spherical vesicles into high-curvature cylinders wrapped in an oligomeric protein coat. In the hetero-complex the adjacent turns of the Dynamin1 helix are separated by 2, 3 or 4 copies of an interleaving molecule of EndophilinA1. Bars 50nm.