Table 1.
Identification codes and components of test samples subjected to the component study. All test samples used for the component study contained AT with a concentration of 0.2 mg/ml. S0 is AT in PBS (at 0.2 mg/ml). ME.0 (1X) and SE (1X) diluted using PBS were used as control samples. S0, M5 and S5 are denoted as AT in PBS, AT in ME.0 and AT in SE, respectively in FTIR, steady-state fluorescence, and HPSEC studies. * For S4, AT was mixed with saturated aqueous solution of DMPC with a concentration lower than 1.1% used in SE.
S0 | AT in PBS (at 0.2 mg/mL) | ||||
---|---|---|---|---|---|
ME.0 component study samples | AT in PBS (0.4 mg/ml) mixed with (1/1, v/v) | ||||
20mM Histidine pH 6.0 | 10% Sucrose | 1% PS80 | 4% Squalene | ||
M1 | + | ||||
M2 | + | + | |||
M3 | + | + | |||
M4 | + | + | + | ||
M5 | + | + | + | + | |
SE component study samples | AT in PBS (0.3 mg/ml) mixed with (2/1, v/v) | ||||
25mM Ammonium Phosphate pH 5.6 | Pluronic F68 0.037% (w/v) | Glycerol 2.37% (w/v) | DMPC 1.1% | 5.1% Squalene 30 μg/mL Vitamin E | |
S1 | + | ||||
S2 | + | + | |||
S3 | + | + | |||
S4 | + | +* | |||
S5 | + | + | + | + | + |