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. 2018 Aug 1;4(4):e262. doi: 10.1212/NXG.0000000000000262

Figure 4. Hypersensitivity of TDP2-mutated patient fibroblasts and A549 cells to DNA damage induced by etoposide but not γ-rays.

Figure 4

(A) Clonogenic survival of 1-BR normal and 850-BR patient primary fibroblasts in a medium containing the indicated concentrations of etoposide. (B) Clonogenic survival of wild-type A549 and TDP2−/− A549 cells in a medium containing the indicated concentrations of etoposide. (C) Clonogenic survival of 1-BR normal and 850-BR patient primary fibroblasts as above, following γ-irradiation. (D), DSBs were measured by γH2AX immunostaining in normal 1-BR and patient 850-BR primary fibroblasts before and after the indicated periods after γ-irradiation (2 Gy). Data are mean ± SEM of 3 independent experiments, and statistically significant differences were determined by two-way ANOVA (ns = not significant, ***p < 0.001). TDP2 = tyrosyl DNA phosphodiesterase 2.