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. 2018 Jun 5;42(3):1445–1459. doi: 10.3892/ijmm.2018.3720

Figure 9.

Figure 9

Liquiritin reduces inflammatory response in UVB-induced HACAT cells in vitro. HACAT cells were pre-treated with liquiritin at 40 and 80 μM concentrations for 2 h, followed by UVB irradiation together for another 22 h. All cells were then harvested and further analysis was performed to evaluate the role of liquiritin in regulating UVB-induced cells in vitro. (A) Pro-inflammatory cytokines IL-1β, TNF-α, IL-18, IL-6 and COX2 were measured using reverse transcription-quantitative polymerase chain reaction analysis. (B) Western blot analysis was performed to evaluate protein levels of TLR4 and MyD88 in vitro. (C) Levels of p-IKKα, IκBα, p-IκBα and p-NF-κB were assessed using western blot analysis. Data are presented as the mean ± standard error of the mean (n=8). ***P<0.001, compared with the Con group. +P<0.05, ++P<0.01 and +++P<0.001, compared with the UVB-only group. UVB, ultraviolet B; Con, control; IL, interleukin; TNF-α tumor necrosis factor-α; COX2, cyclooxygenase 2; TLR4, toll-like receptor 4; MyD88, myeloid differentiation factor 88; NF-κB, nuclear factor-κB; IκBα, inhibitor of NF-κB α; IKKα, IκB kinase α; p-, phosphorylated.