Table I.
Sequences of primers used in reverse transcription-quantitative polymerase chain reaction analysis.
| Gene | Primer sequence (5′-3′) |
|---|---|
| Mouse-TNF-α | F: AGATATTACAGTGCCATGGTT |
| R: ACGAGTAGAGATCTCGAG | |
| Mouse-COX2 | F: CCAGACTTGGCTACCTCGTG |
| R: CTGACTTAGTCGATAATTCT | |
| Mouse-SOD1 | F: GCCATTCCTGTCTCGTGGAG |
| R: TAATGTATTGATCCTTGATTAT | |
| Mouse-IL-18 | F: AGTAAGTGGCATTACCGAC |
| R: ACCAACGCAACAGTCTGCAG | |
| Mouse-IL-6 | F: GTCAAGACCACAGCTAGC |
| R: CTGGCGACTACTAGTAGATA | |
| Mouse-IL-1β | F: ACTCGAGAGGAATCCTTACGA |
| R: CACTCTCGTGACTCGCTA | |
| Mouse-CAT | F: CCTCGTTCACACTTCGTGTA |
| R: GGAAGCACTCCAGTGCAGC | |
| Mouse-Nrf2 | F: CTACAGACCAATGCCTGAC |
| R: ACGTAACTGTGCCATGGGGA | |
| Mouse-SOD2 | F: AGGCCGTGAGAGCTTGTGTA |
| R: CGGCAGTAAGTGCCCTCTAC | |
| Mouse-GAPDH | F: GCGAGCTGAGACACTCTAG |
| R: TAGGCATGCACCTCTGTTCA | |
| Human-TNF-α | F: ATATGTGCTGGTCATACTCAT |
| R: AGAGATCGGCGTCAGATGA | |
| Human-COX2 | F: CCTTGCAGAGCTACCGAGT |
| R: GTGACTTAGTATATTCATCC | |
| Human-SOD1 | F: GTCCTACGGTCTCGTGAGATAT |
| R: GTTAATATTGGTAGTTCTCTG | |
| Human-IL-18 | F: AGCATTACCGACCTATTCCT |
| R: AGCACCCAACGCAACAAGTG | |
| Human-IL-6 | F: CAGTAGAAGACCAAGTACAT |
| R: CTGGCGACTACTTAGCTATAA | |
| Human-IL-1β | F: AATTGAGAGCTCGGATCCGT |
| R: CTGACGCTATCACGTAGAGCA | |
| Human-CAT | F: CTCACTACGTCGTGTATTCCCT |
| R: GTGAAGCACTCCAGTGCCAG | |
| Human-Nrf2 | F: CTCCAATGCCTGATCGCGCT |
| R: AGGCCATGGCAAGTGTGAT | |
| Human-SOD2 | F: CGTGAGGCGAGCTACAGAA |
| R: GGTAAGTTACGCTCCAGCCTC | |
| Human-GAPDH | F: GGACCGAGTACTGCATCAGCTA |
| R: TCCTCGTGAAGGTTGACTGCGA |
TNF-α, tumor necrosis factor-α; COX2, cyclooxygenase 2; SOD, superoxide dismutase; IL, interleukin; CAT, catalase; Nrf2, nuclear factor erythroid 2-related factor 2; F, forward; R, reverse.