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. 2018 Jun 8;42(3):1695–1715. doi: 10.3892/ijmm.2018.3725

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Copyright: © Bahman et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Flow cytometric analysis of apoptosis in human hepatic cancer cell lines treated with Sora, Kmf and their simultaneous combinations. (A) Hep3b and (B) HepG2 hepatic cancer cell lines were (a) left untreated or treated with (b) Sora (5 μM), (c) Kmf (200 μM), (d) Kmf (400 μM), or (e and f) simultaneous combinations of Sora and Kmf (5 μM + 200 μM, respectively) or (5 μM + 400 μM, respectively) for 48 h. The cells were analyzed by flow cytometry after processing and staining with Annexin V-FITC/PI. B1, percentage of necrotic cells; B2, percentage of late apoptotic cells; B3, percentage of viable cells; and B4, percentage of early apoptotic cells. Sora, sorafenib; Kmf, kaempferol; UT, untreated; PI, propidium iodide; FITC, fluorescein isothiocyanate.