Figure 3.

ROS generation in melanoma cells was increased due to resveratrol exposure. (A) Intracellular ROS production in the A375SM cell line was measured using a DCF-DA assay. A375SM cells were treated with DMSO (control) or resveratrol (1.0 and 10 µM) for 48 h. Cellular ROS production was observed using a fluorescence microscope. (B) The amount of ROS formed by resveratrol treatment was quantified using CellSens Dimension software. Data of three single experiments are represented as the mean ± standard deviation. ^*P<0.05 vs. control (0.1% DMSO). Res, resveratrol; DMSO, dimethyl sulfoxide; ROS, reactive oxygen species; DCF-DA, 2′,7′-dichlorofluorescein diacetate.