Figure 5.

Kushui Rose decoction (KRD) decreased significantly the level of reactive oxygen species (ROS) accumulation in let-60(gf) mutants. (A) Fluorescence image of worms stained by H₂DCF-DA, which were stained by 10 µM H₂DCF-DA at 20°C for 20 min (N=20). Con and C, control. NAC, 2.5 mM N-Acety-L-Cysteine; PQ, 0.5 mM paraquat. Scale bar, 200 µm. (B) Quantified fluorescence intensity of H₂DCF-DA of each group. 2.5 mM NAC and 0.5 mM PQ were used as positive and negative control, respectively. Data are presented as the mean ± SD of at least three independent experiments. Bars with different letters indicate that there was a significant difference at a level of 0.05 among groups.