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. 2018 Aug 6;28(15):2365–2376.e5. doi: 10.1016/j.cub.2018.05.068

Figure 7.

Figure 7

Physcomitrella CLE Peptides Act via a Conserved Receptor Machinery

(A and B) Treatment of Arabidopsis seedlings with 1 μM CLV3-like CLEs from Arabidopsis and Physcomitrella, but not TDIF-like CLEs, suppresses root meristem proliferation in WT Arabidopsis (n = 30; ANOVA; Tukey’s HSD; p < 0.0005). The scale bar in (A) represents 1 cm and scale bar in (B) represents 100 μm; black bars in (B) represent approximate position and extent of root meristem.

(C and inset in A) Arabidopsis rpk2-4 mutants are resistant to treatment with 1 μM CLE peptides; n ≥ 15; ANOVA; Tukey’s HSD; p < 0.0005. The scale bar represents 100 μm in (C).

(D) Gametophores in plants treated with 10 μM CLEs are stunted in WT, PpcleAmiR1-3, and PpcleAmiR4-7 mutants, but not Pprpk2 mutants (n ≥ 20; ANOVA; Tukey’s HSD; p < 0.0005). The scale bar represents 100 μm.

(E and F) Quantitative data on root length in WT (E) and rpk2 mutant plants (F), supporting inferences from images shown in (A).

(G–J) Quantitative data on gametophore length in WT (G), PpcleAmiR1-3 (H), PpcleAmiR4-7 (I), and Pprpk2 (J), mutant plants supporting inferences from images shown in (D).