Fig. 1.

Induction of Ablim1 expression during RANKL-mediated osteoclastogenesis. BMMs were cultured with M-CSF (30 ng/ml) and RANKL (100 ng/ml) for the indicated times. The mRNA (A) and protein (B) expression level of ABLIM1 was determined by RT PCR and Western blot analysis, respectively. The relative expression level was measured using Gapdh and Actin as loading controls (lower panels). Data are expressed as mean ± SD from triplicate experiments. NS, not significant. *P < 0.05, **P < 0.01 versus control (Day 0).