Fig. 3.

Effects of ABLIM1-specific knockdown on expression of osteoclast differentiation marker genes and transcriptional factors. Control vector (shGFP)- or shAblim1-infected BMMs were cultured with M-CSF (30 ng/ml) and RANKL (100 ng/ml) for indicated times. (A) The relative mRNA expression levels of osteoclast differentiation marker genes were measured by real-time PCR and normalized to the expression of Gapdh. (B) c-Fos and NFATc1 expression levels were detected by anti-c-Fos and anti-NFATc1 antibodies, respectively, and normalized to the actin level. Data are expressed as the representative mean ± SD from at least three independent experiments. *P < 0.05, **P < 0.01 versus control (shGFP).