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. 2018 Aug 13;4:82. doi: 10.1038/s41420-018-0084-z

Fig. 5. Attenuation of the inhibitory effects of SC-43 and SC-78 on sphere-forming HCT-116 and HT-29 cells by SHP-1 knockdown.

Fig. 5

a Total lysates (20 μg) were prepared from two different SHP-1 knockdown clones (SHP-1 shA and SHP-1 shB) derived from HCT-116 (left) and HT-29 (right) cells and subjected to western blot analyses using an anti-SHP-1 antibody as a probe. GAPDH was used as a loading control. b Parental (WT) HCT-116 cells and their SHP-1 knockdown clones (left) as well as parental HT-29 cells and their SHP-1 knockdown clones (right) were cultured in defined media. After 7 days, spheres stained by MTT were photographed (scale bar = 0.7 cm), and their numbers were quantified by MetaMorph software. Data (lower panels) are the mean ± SD of three independent experiments (N.S. indicates no significant difference compared with parental cells by Student’s t-test). c Parental HCT-116 cells and their SHP-1 knockdown clones (SHP-1 shA) (left) as well as parental HT-29 cells and their SHP-1 knockdown clones (SHP-1 shB) (right) were cultured in defined media containing DMSO (vehicle), 0.5 μM SC-43, or 0.4 μM SC-78 for 7 days. The sphere number was quantified. *p < 0.05, **p < 0.01, ***p < 0.005, and ****p < 0.001 compared with DMSO-treated cells by Student’s t-test