Table 3.
Model validation demonstrates identifiability of subpopulation compositions: Measured versus two population model estimated parameters indicate the model’s ability to reveal the known proportions of the MCF-7/ADR resistant cell line mixed with the wild-type MCF-7 cell line.
| Measured | Model | |
|---|---|---|
| LD50ADR | 187.5 +/− 3.8 | 186.7 +/− 4.6 |
| slopeADR | 0.034 +/− 0.0039 | 0.028 +/− 0.0028 |
| LD50WT | 37.1 +/−3.3 | 35.95 +/− 2.8 |
| slopeWT | 0.055 +/− 0.0077 | 0.060 +/− 0.0076 |
| frac0%ADR | 0.07 +/− 0.01 | 0.05 +/− 0.03 |
| frac25%ADR | 0.30 +/− 0.04 | 0.44 +/− 0.04 |
| frac50%ADR | 0.54 +/− 0.05 | 0.075 +/− 0.04 |
| frac75%ADR | 0.83 +/− 0.05 | 0.91 +/− 0.04 |
| frac100%ADR | 1.0 +/− 0.02 | 1 +/− 0.0272 |
“Measured” LD50 values and slopes indicate the LD50 and slope of the isolated pure MCF-7/ADR cells and pure wild-type MCF-7 cells fit to the single static population model. Measured resistant fractions are measured precisely using the Nexcelom fluorescence imaging counter of the number of green fluorescent wild-type MCF-7 compared to the total number of brightfield cells counted.