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. 2018 Aug 13;8:12028. doi: 10.1038/s41598-018-30537-z

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Detection of cTNT, α-MHC, α-SA and Cx43 in ^{Cardiopoietic}AF cells and in CM-like cells obtained after 15 days of differentiation. (a) Flow cytometry analysis of TnT, α-MHC, α-SA and Cx43 in ^{Cardiopoietic}AF cell (blue line) and CMs (red line). (b) Immunofluorescent staining for TnT, α-MHC, α-SA and Cx43 (green), as indicated. The nuclei are counterstained with DAPI (blue). Note the typical sarcomeric striations of α-SA. Red arrows indicate Cx43 localization on the cell membrane. Scale bar: 10 μm. (c) Western blot analysis of the cardiac markers. Human heart and fibroblast lysates were used as controls. The G6PDH lane is used as a loading control. Data are representative of seven independent experiments.