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. 2018 Aug 13;8:12028. doi: 10.1038/s41598-018-30537-z

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Detection of the atrial marker MYL7 and of the ventricle-specific nuclear factor IRX4 in ^{Cardiopoietic}AF cells and in CM-like cells obtained after 15 days of differentiation. (a) Flow cytometry and (b) Western blot for MYL7 and IRX4 in ^{Cardiopoietic}AF cells and CMs. In Western Blot human heart and fibroblast lysates were used as controls. In the histogram of the cytometric analysis, the gray line represents the isotype control, the blue line represents ^{Cardiopoietic}AF cells and the red line represents CMs. (c) Merged immunofluorescent staining for MYL7 (green) and IRX4 (red) and nuclei (blue) in ^{Cardiopoietic}AF cells (left panel) and in CMs (right panel). Red arrows indicate the nuclear localization of IRX4 in the differentiated cells. Scale bar: 10 μm. Data are representative of seven independent experiments.