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. 2018 Aug 13;8:12028. doi: 10.1038/s41598-018-30537-z

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Intracellular Ca²⁺ variations and expression of RyR and β receptors in ^{Cardiopoietic}AF cell-derived CM-like cells. (a) Spontaneous Ca²⁺ oscillations recorded in ^{Cardiopoietic}AF cell-derived CMs. (b) CM intracellular Ca²⁺ variations in response to 50 mM KCl and 40 mM caffeine in the presence or absence of extracellular calcium (NES calcium free). (c) Flow cytometry of RyR expression. Gray line: isotype control; blue line: ^{Cardiopoietic}AF cells; red line: CMs. (d) Western blot for β ₁AR and β ₂AR in ^{Cardiopoietic}AF cells and CMs. Human heart and fibroblast lysates were used as controls. (e) Effect of 1 M isoproterenol (Iso) on 50 mM KCl-induced depolarization in CM. Data are representative of 7 different experiments.