Table 1.

Characterization of AF cells samples.

AF Sample	Age at pregnancy (yr)	Time of Amniocentesis (Weeks of Gestatiion)	Fetal Karyotype	AF cell phenotyping (%)			EBs formation
				SSEA4	OCT4	CD90
#1	38	16	46, XY	65.3	60.2	84.1	+
#2	38	16	46, XY	9.8	13.5	50.1	−
#3	37	16	46, XY	9.9	3.8	9.1	−
#4	38	16	46, XY	54.5	15.4	93.5	+
#5	40	16	46, XY	82.2	73.7	48.1	+
#6	40	17	46, XY	n.d.	9.9	n.d.	−
#7	42	16	46, XY	5.7	6.7	43.3	−
#8	41	16	46, XY	6.3	2.4	10.2	−
#9	39	16	46, XY	80.4	12.5	64.6	+
#10	35	17	46, XY	59.1	78.5	45.6	+
#11	41	16	46, XY	25.1	n.d.	7.8	−
#12	38	16	46, XY	9.9	6.8	51.3	−
#13	38	16	46, XY	6.8	7.4	5.7	−
#14	32	17	46, XY	63.9	12.1	90.0	+
#15	35	17	46, XY	56.8	82.7	81.1	+

In bold are highlighted the AF cells samples (^{Cardiopioetic}AF cells) able to give rise to EB-like aggregates.

n.d.: not detectable.