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. 2018 Aug 13;8:12005. doi: 10.1038/s41598-018-30511-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Fluorescent images of KYSE220 cells stained with sc-120 or sc-59906, and Cy3 anti-mouse IgG antibody. The scale bar indicates 100 μm. (B) Flow cytometry analysis of KYSE220 cells stained with sc-120 or sc-59906, and Alexa Fluor 488-labeled anti-mouse IgG antibody. The x-axis shows logarithmic fluorescence intensity. Mean fluorescence intensity (MFI) was calculated from the flow cytometry data.