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. 2018 Aug 13;8:12074. doi: 10.1038/s41598-018-30501-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Experimental setup for the structural tomographic imaging of the nervous system. (a) Schematic view of the experimental setup for synchrotron-based X-ray microtomography. (1) X-rays from a synchrotron bending magnet source illuminated the samples positioned in front of an indirect X-ray detector (2). (3) Several projection images were acquired by a rotational scan and used to digitally reconstruct the tomographic slices (4). (b) Segmentation of one mouse brain hemisphere, showing the brain cortex (green), midbrain (blue) and cerebellum (red).