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. 2018 Aug 13;8:12074. doi: 10.1038/s41598-018-30501-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Synchrotron X-ray microtomography of mercury-impregnated neurons allows virtual slicing and 3D rendering of whole brain structures. Reconstructed slice (left panel) and 3D image rendering (right panel) of a control frontal cortex (a) and hippocampus (b). Rectangles in (a) and (b) highlights, respectively, a single cortical or hippocampal neuron. Arrowheads: neuron cell bodies; arrows: neurites; red circles: longitudinal virtual slicing of a neurite; yellow circles: transversal virtual slicing of a neurite.