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. 2018 Aug 13;8:12079. doi: 10.1038/s41598-018-30621-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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D18 augmented T regs expansion and maintained their suppressive function in vitro. (a) T regs were expanded for two weeks under two conditions, ‘UT’: with IL2 and anti-CD3 coated Dynabeads; ‘D18’: the UT condition plus D18. Adding D18 to the culture system significantly increased the number of T regs. (*p = 0.031, data were generated from 3 separate experiments). (b) A representative suppression assay was shown. T regs expanded under the two conditions showed the same capacity to suppress T cons proliferation under two different cell to cell ratios. Data were generated from 3 separate experiments.