Fig. 7.
Decreased active-beta catenin staining of bone surfaces from Rspo2-deficient mice. a, b Representative images of active beta-catenin immunohistochemistry of bones from 3-month-old mice. Arrows indicate bone surfaces and trabecular bone is labeled. Red indicates cell-associated active beta-catenin presence on trabecular bone (a), WT, (b). Ocn-Cre + Rspo2f/f Scale bar is 100 μmol·L-1. g, h Histomorphometric quantification of beta-catenin positive bone surfaces. c Number of active-beta catenin positive cells. d Beta-catenin-positive surface. e Beta-catenin positive surface relative to total bone surface. f Percent Ocn-Cre + Rspo2f/f active-beta catenin positive cells relative to WT. g Percent Ocn-Cre + Rspo2f/f-positive active-beta catenin surface relative to WT. h Percent positive Ocn-Cre + Rspo2f/f active-beta catenin surface per total surface. WT n = 3 (2 male, 1 female), Ocn-Cre + Rspo2f/f, n = 3 (2 male, 1 female). *indicates P < 0.05 for genotype groups