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. 2018 Aug 13;8:12042. doi: 10.1038/s41598-018-30525-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) The endocytosis effectiveness of dextran-FITC particles by stimulated DCs. Lysates and TCM used in these analyses were collected from cultures of HCT116 and HT29 CRC lines expanded in adherent or spherical forms (allogenic). Data are presented as fold increase of the proportions of dextran-FITC⁺ cells in comparison to iDCs after incubation with dextran-FITC particles. The control value was set at the level of 1. Bars and whiskers represent mean ± SEM (*p < 0.05 vs iDCs, ^•p < 0.05 lysate vs lysate/LPS or TCM vs TCM/LPS, ANOVA Kruskal – Wallis test, n = 15). (B) The viability of HCT116 cells after 24 h co-culture with stimulated DCs. The fold increase of CD133⁺7AAD⁺cells proportions was determined by flow cytometry by comparison of stimulated DCs with iDCs. The control value was set at the level of 1. Bars and whiskers represent mean ± SEM (*p < 0.05 vs iDCs, ^•p < 0.05 lysate vs lysate/LPS or TCM vs TCM/LPS, ANOVA Kruskal – Wallis test, n = 15).